Sec Form 4/A Filing - DEMSKI MARTHA J @ Alpha Teknova, Inc. - 2025-06-17

FORM 4/A Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940

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1. Name and Address of Reporting Person DEMSKI MARTHA J 2. Issuer Name and Ticker or Trading Symbol Alpha Teknova, Inc. [ TKNO] 5. Relationship of Reporting Person(s) to Issuer (Check all applicable) X __ Director _____ 10% Owner _____ Officer (give title below) _____ Other (specify below) (Last) (First) (Middle) C/O ALPHA TEKNOVA, INC., 2451 BERT DRIVE 3. Date of Earliest Transaction (MM/DD/YY) 06/17/2025 (Street) HOLLISTER, CA95023 4. If Amendment, Date Original Filed (MM/DD/YY) 06/30/2025 6. Individual or Joint/Group Filing (Check Applicable Line) __ X __ Form filed by One Reporting Person _____ Form filed by More than One Reporting Person (City) (State) (Zip)

Table I - Non-Derivative Securities Acquired, Disposed of, or Beneficially Owned 1.Title of Security (Instr. 3) 2. Transaction Date (MM/DD/YY) 2A. Deemed Execution Date, if any (MM/DD/YY) 3. Transaction Code (Instr. 8) 4. Securities Acquired (A) or Disposed of (D) (Instr. 3, 4 and 5) 5. Amount of Securities Beneficially Owned Following Reported Transaction(s) (Instr. 3 and 4) 6. Ownership Form: Direct (D) or Indirect (I) (Instr. 4) 7. Nature of Indirect Beneficial Ownership (Instr. 4) Code V Amount (A) or (D) Price

Table II - Derivative Securities Acquired, Disposed of, or Beneficially Owned ( e.g. , puts, calls, warrants, options, convertible securities) 1. Title of Derivative Security (Instr. 3) 2. Conversion or Exercise Price of Derivative Security 3. Transaction Date (MM/DD/YY) 3A. Deemed Execution Date, if any (MM/DD/YY) 4. Transaction Code (Instr. 8) 5. Number of Derivative Securities Acquired (A) or Disposed of (D) (Instr. 3, 4, and 5) 6. Date Exercisable and Expiration Date (MM/DD/YY) 7. Title and Amount of Underlying Securities (Instr. 3 and 4) 8. Price of Derivative Security (Instr. 5) 9. Number of Derivative Securities Beneficially Owned Following Reported Transaction(s) (Instr. 4) 10. Ownership Form of Derivative Security: Direct (D) or Indirect (I) (Instr. 4) 11. Nature of Indirect Beneficial Ownership (Instr. 4) Code V (A) (D) Date Exercisable Expiration Date Title Amount or Number of Shares Non-Qualified Stock Option (right to buy) $ 5.39 06/17/2025 A 54,300 ( 1 ) 06/17/2026 Common Stock 54,300 $ 0 54,300 D

Reporting Owners Reporting Owner Name / Address Relationships Director 10% Owner Officer Other DEMSKI MARTHA J C/O ALPHA TEKNOVA, INC. 2451 BERT DRIVE HOLLISTER, CA95023 X Signatures By: Damon A. Terrill For: Martha Demski 06/30/2025 Signature of Reporting Person Date

Explanation of Responses:

( 1 )On June 25, 2025, a Form 4 was filed on behalf of the reporting person that incorrectly identified the type of securities granted to such person on June 17, 2025 as being 54,300 restricted stock units, which were included in Table I. This amended Form 4 is being filed to correct that the securities granted to the reporting person on June 17, 2025 were non-qualified stock options to purchase 54,300 shares of common stock, which were granted under the issuer's director compensation policy. These non-qualified stock options are identified on Table II and will vest on the first anniversary of the date of grant.

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4(b)(v).

** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).

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